Efficacy of bioagents, botanical and fungicides against Fusarium oxysporum f.sp. baisilici under in Vivo condition

Abstract

Tulsi is native to Indian subcontinent and wide spread as a cultivated plant throughout the south-east Asian tropics. The Holy Basil (Ocimum sanctum L.) commonly known as Tulsi, is an aromatic perennial plant, belongs to the family Lamiaceae. Holy Basil is widely grown in India for medicinal as well as religious purposes. For the management of wilt disease of Holy Basil (Ocimum sanactum) the experimental finding depicted in Table no.3 reveal that among 6 treatment which include three bio-agent T1 Trichoderma viride, T2 Trichoderma harzianum and T3 Pseudomonas fluorescence and one botanicals T4 Neem leaves and two chemicals T5 Tebuconazole and T6 Mancozeb. The most effective was Tebuconazole 250 EC @ 0.1% with percent disease control and percent disease intensity, (68.20) and (19.67) respectively. and Mancozeb 75WP @ 0.1%. with PDC and PDI (62.54) and (23.17), respectively whereas the bio-agent Trichoderma viride Talc based formulation was prepared.cfu-1×109/gm and dissolved @ 10gm in 1.0 lit. distilled water) with PDC and PDI (45.41) and (33.75), respectively. Trichoderma harzianum (Talc based formulation was prepared.cfu-1×109/gm and dissolved @ 10gm in 1.0 lit. distilled water) with PDC and PDI (37.02) and (38.94) respectively and Pseudomonas fluorescence @ 4 gm/kg seed with PDC and PDI (27.76) and (44.62) respectively. and Neem leaves @ 5% with PDC and PDI (16.98) and (51.53).The used fungicide and bio pesticide significantly reduce the wilt of Ocimum sanctum.